產(chǎn)品詳情介紹
ProteinIso? Ni-NTA Resin是螯合金屬Ni2+而形成的一種親和層析介質(zhì)。NTA能夠通過四個(gè)位點(diǎn)牢固地螯合Ni2+，從而減少純化過程中Ni2+泄漏到蛋白樣品中。本產(chǎn)品對(duì)His標(biāo)簽蛋白具有特異吸附能力，從而使His標(biāo)簽蛋白結(jié)合在Ni-NTA純化介質(zhì)上，未結(jié)合的蛋白被洗滌下去。結(jié)合在介質(zhì)上的蛋白經(jīng)過一定濃度的咪唑或者pH低緩沖液溫和洗脫下來，從而得到高純度的目的蛋白。本產(chǎn)品具有吸附容量大、選擇性好、通透性強(qiáng)、易于再生等優(yōu)點(diǎn)，適用于在非變性或變性條件下純化任何表達(dá)系統(tǒng)表達(dá)的His標(biāo)簽重組蛋白。
1 Wang F P, Zhao P P, Zhang L, et al. The VvWRKY37 Regulates Bud Break in Grape Vine Through ABA-Mediated Signaling Pathways[J]. Frontiers in Plant Science, 2022.(IF 6.62)
2 Liu Y, Yu T F, Li Y T, et al. Mitogen‐activated protein kinase TaMPK3 suppresses ABA response by destabilising TaPYL4 receptor in wheat[J]. New Phytologist, 2022.(IF 10.32)
3 Guo L, Zhao Y, Zhang Q, et al. Stochastically multimerized ParB orchestrates DNA assembly as unveiled by single-molecule analysis[J]. Nucleic Acids Research, 2022.(IF 19.16)
4 Zhang Y, Gao Y, Wang H L, et al. Verticillium dahliae secretory effector PevD1 induces leaf senescence by promoting ORE1-mediated ethylene biosynthesis[J]. Molecular plant, 2021.(IF 13.16)
5 Zhang Q, Chen Z, Wang F, et al. Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer[J]. Nucleic Acids Research, 2021.(IF 16.97)
